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| image credit: medical.escoglobal.com |
"There are many differences between Stockholm, Denver and Lhasa. One of them is the need for different CO2 settings in the IVF lab, due to different altitudes. In this blog post Dr. Markus Montag gives advice on how to ensure successful embryo culture at high altitude.
Altitude makes a difference
Not all IVF laboratories have the luxury to be located at a nice setting at sea level with beaches just outside the door. I guess most of us would consider these fellow-embryologists to be the lucky ones. And they are, not only because of the life-style benefits, but also because they clearly have an easy task in setting the CO2 value in their incubators in order to have the proper pH in their culture media.
Why so? Firstly, because they do not necessarily need to know much about the Henderson-Hasselbalch equation. You may remember this formula from chemistry lessons at school or at university or of one of these questions asked during embryology certification. The Henderson-Hasselbalch equation describes the relation between the pH-value and the reaction between acidic and basic components in a solution..."
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